Zooplankton samples were collected onboard the research Icebreaker CCGS Amundsen during Leg 1 in 2005 (4 stations) and Leg 1 in 2006 (8 stations) with a vertically towed net frame equipped with a set of 4 adjacent 1-m2 nets (mesh size 200 µm and 500 µm) from near the bottom to the surface and by trawling an oblique rectangular mid-water trawl (mesh size 1600 µm) or an oblique Tucker net (mesh size 2x500 µm) in the surface layer from 100 m depth to the surface. Samples were placed into 30 ml plastic vials and/or whirl-pak bags and were kept frozen at -20 Degree Celcius. Representative sub-samples of individual zooplankton genera were placed in 4 ml glass vials for stable isotope analysis. Three keystone zooplankton genus were included in this study: Calanus spp. (mostly adult Calanus hyperboreus), Themisto spp. (mostly adult Themisto libellula) and Euchaeta spp. THg analysis was conducted at the Freshwater Institute with cold vapour atomic absorption spectroscopy (CVAAS). MMHg analysis was conducted at the University of Ottawa by Gas Chromatography Atomic Fluorescence Spectroscopy (GCAFS). Carbon and nitrogen isotopic analyses were performed at the University of Winnipeg Isotope Laboratory by continuous flow ion ratio mass spectrometry (CF-IRMS). Oceanographic data (Hg levels in water column, salinity, 18O) were collected by our team. Water samples were collected with 24 12-L Niskin bottles attached to a rosette sampler equipped with Seabird 911+ CTD (Sea-Bird)

Ocean Mapping Group acoustic data generated from the 2003 multibeam sonar survey on the 2003 CCGS Amundsen ArcticNet Cruise. The data include high-resolution bathymetry and acoustic backscatter imagery collected 24 hours a day, whenever the ship was in transit or had dedicated survey time.

During the ArcticNet annual cruises of the research icebreaker CCGS Amundsen, characteristics of the near-surface atmosphere (basic meteorological elements, incident radiation, CO2 concentration) are monitored in conjunction with surface sea water properties (temperature, salinity, dissolved CO2 and O2) to observe the relationship between the surface micro-climate and the air-sea exchange, with particular interest in CO2. As part of this integrated dataset, the following radiation variables were recorded at 1 minute intervals (instrument used to collect each variable is in parentheses): incoming shortwave radiation (Eppley pyranometer, model PSP); incoming longwave radiation (Eppley pyrgeometer, model PIR); incoming photosynthetically active radiation (Kipp & Zonen, PAR-Lite); and, incoming UVa,b (Kipp & Zonen, UVS-AB-T). All instruments were mounted on a meteorological tower on the bow of the research icebreaker CCGS Amundsen.

The meteorological observatory Polarstern continuously acquires meteorological parameters during times of ship operation. Measurements are taken on various locations on the vessel, instrument heights above sea level are given below. All data is quality controlled. Measurements are checked daily on board by the operator and again prior to publication. Knowingly affected or erroneous data is removed.

During the ArcticNet annual cruises of the research icebreaker CCGS Amundsen, characteristics of the near-surface atmosphere are monitored in conjunction with surface sea water properties to observe the relationship between the surface micro-climate and the air-sea exchange , with particular interest in CO2. Central to this integrated dataset is an eddy covariance system used to monitor fluxes of CO2, H2O, heat and momentum. The system continuously sampled the following variables at a rate of 10 Hz (instrument used to collect each variable is in parentheses, and approximate instrument height above surface is indicated): -3D wind velocity (Gill R3 and Gill Windmaster Pro ultra-sonic anemometer) - 15m -Sonic air temperature (Gill R3 and Gill Windmaster Pro ultra-sonic anemometer) - 15m -CO2 molar concentration (LI7500 open path gas analyzer) - 15m -H2O molar concentration (LI7500 open path gas analyzer) - 15m -CO2 mixing ratio (LI7000 integrated into a closed path system) - 15m -H2O mixing ratio (LI7000 integrated into a closed path system) - 15m -3D ship motion - angular rates and accelerations (MotionPak, Systron Donner) - 13m All instruments were mounted on a meteorological tower on the bow of the research icebreaker CCGS Amundsen.