A total of 19 stations were sampled between August and October 2011 onboard the Canadian research icebreaker CCGS Amundsen. Two stations were sampled both in August and October to assess seasonal variability in stable isotope composition. At each station, a USNEL box corer (0.25 m2) was deployed to collect seafloor sediments for the determination of stable isotope composition and pigment (Chl a + phaeopigments) concentrations. From each box core, surface sediments (upper 1 cm) were sampled as three sub-cores using a 60 ml disposable syringe (2.6 cm diameter with a cut off anterior end). Sediment samples were immediately frozen at -20 °C for stable isotope analysis and at -80 °C for pigment analysis. Megabenthic invertebrates were principally collected with an Agassiz trawl (effective opening of 1.5 m and a net mesh size of 40 mm, with a 5 mm cod end liner) with average trawling time and speed of 5 min and 1.5 knots, respectively. At three stations, invertebrates were collected with the box corer. Trawl and box corer catches were washed over a 2 mm sieve under running sea water onboard and 1 to 3 individuals of the most abundant community representatives were collected at each station. Specimens were frozen immediately at -80 °C and identified to the lowest possible taxonomic level in the lab.

Microbial and environmental variables were collected from 8 depths at a 200-m deep site in Franklin Bay on 33 occasions, from 4 November 2003 to 6 August 2004, aboard the CCGS Amundsen. The following variables were measured: depth, temperature and salinity (Seabird 911+ CTD); CDOM (coloured dissolved organic matter) absorption coefficient at 320 nm (Varian Cary Bio 300 scanning spectrophotometer); chlorophyll a (ethanol pigment extraction); bacteria abundance (epifluorescence microscopy); tritiated leucine and thymidine incorporation rates (centrifugation method). Bacterial carbon production (BP) was estimated from leucine incorporation using the carbon conversion factor of 1.5 kgC/mol of leucine incorporation. BP was also estimated from thymidine incorporation using (1) the empirical carbon conversion factor of 2.0 x10^18 cells/mol of thymidine incorporated and (2) the bacterial cellular biomass of 10 fgC/cell.

The study area extends from the North Water Polynya to the Chukchi Sea across five geographic regions: North Water Polynya, Canadian Archipelago, Amundsen Gulf, Beaufort Sea and Chukchi Sea (14 stations). We collected data from July to October 2014 aboard the CCGS Amundsen including environmental data (bottom water temperature and salinity), particulate organic matter, sediments, and benthos. Stable isotopes analyses were practiced on benthic samples, particulate organic matter samples and sediments samples. Lipid extraction and ice algae biomarker analysis were practiced on benthic samples and sediments samples.

Membrane inlet mass spectrometer (MIMS) and optode / gas tension device (GTD) gas systems were deployed on the CCGS Amundsen in 2018 and 2019. These autonomous sensors obtained measurements of dissolved O2, Ar, and N2 from the ship's underway seawater supply line at approximately 10-20 sec. intervals (<100 m spatial resolution). Raw and calibrated absolute gas concentrations, supersaturation anomalies, and gas ratios are reported. Data from the underway gas instrumentation were combined with hydrographic observations of surface and depth-resolved temperature and salinity (obtained by the Amundsen Science research group) collected via underway thermosalinograph (TSG) and Rosette-mounted conductivity-temperature-depth (CTD) instrumentation.