• ARICE Metadata Catalogue
  •  
  •  
  •  

HPLC Pigment Analysis of the Phytoplankton Community in Franklin Bay

Sample collection: The sampling was part of the Canadian Arctic Shelf Exchange Study (CASES) in which CCGS Amundsen was frozen in Franklin Bay in the coastal Beaufort Sea (Station FB/200) during winter. Upper mixed-layer microbial plankton communities were sampled 5m below the water surface using the ship CTD rosette system equipped with 12L Niskins during openwater conditions. During the time that the ship was frozen in Franklin Bay from December 2003 to early June 2004, samples were taken using a 5L Niskin bottle from 3m below the bottom ice through a 300mm hole that had been drilled 500m upstream of the ship. HPLC pigment analysis: One to two liter samples of water were filtered onto Whatman GF/F filters and stored frozen at -80C until analysis. Phytoplankton pigments on the GF/Fs were extracted in 3mL of 95 percent MeOH and 100 uL of the extracts was injected into a Varian ProStar HPLC equipped with a Symmetry C8 column. The HPLC peaks were detected by diode-array spectroscopy (350-750nm) and absorbance chromatograms were obtained at 440 (for chls) and 450nm (for carotenoids). Chlorophylls were also detected by fluorescence (excitation: 440nm; emission: 650nm). The HPLC solvent protocol was based on gradient dilution with two solvent mixtures (Zapata et al. 2000): a methanol, acetonitrile, and aqueous pyridine (50:25:25 v:v:v) solution; and a methanol, acetonitrile, and acetone (20:60:20 v:v:v) solution. The flow rate was 1mL/ min, and the equilibration time was 7min.

Simple

Date (Publication)
2016-01-14
Principal investigator
 

Vincent, Warwick


Originator
 

Martineau, Marie-Josée


Other citation details

Lovejoy, C., Vincent, W.F., Bonilla, S., Roy, S., Martineau, M.-J., Terrado, R., Potvin, M., Massana, R. and Pedrós-Alió, C. 2007. Distribution, phylogeny and growth of cold-adapted picoprasinophytes in arctic seas. J. Phycol. 43, 78-89.


Matsuoka, A., Larouche, P., Poulin, M., Vincent, W. and Hattori, H. 2009. Phytoplankton community adaptation to changing light levels in the southern Beaufort Sea, Canadian Arctic, Estuarine, Coastal and Shelf Sciences 82: 537-546.

Purpose

To obtain a detailed description of the pigments associated with the phytoplankton community. The pigment analyses from the year-long study in the coastal Beaufort Sea of the western Canadian Arctic showed the continuous prevalence of pigments associated with eukaryotic picoplankton in the green algal class Prasinophyceae. These results confirmed the presence of Micromonas observed by microscopy.

Status
Completed
Point of contact
 

Canadian Cryospheric Information Network

-

Polar Data Catalogue


200 University Avenue West, University of Waterloo

,

Waterloo

,

Ontario

,

N2L 3G1

,

Canada

(519) 888-4567 x32689
polardata.ca

Polar Data Catalogue Thesaurus (Canada)

  • Arctic

  • Chlorophyll

  • Franklin Bay

  • Phytoplankton

  • Pigments

Place
  • Franklin Bay, Northwest Territories, Canada (LAIQA)

Access constraints
Other restrictions
Use constraints
Other restrictions
Other constraints

Terms of Use of the Polar Data Catalogue: https://www.polardata.ca/pdcinput/public/termsofuse

Metadata language

eng; CAN

Topic category
  • Environment
N
S
E
W
thumbnail


Begin date
2003-10-19
End date
2004-08-06
Supplemental Information

Summary: Not Applicable Research Program(s): CASES. For further information: warwick.vincent@bio.ulaval.ca warwick.vincent@bio.ula Summary: Not Applicable Research Program(s): CASES. For further information: warwick.vincent@bio.ulaval.ca

Distribution format
  • Digital file

    (

    1.0

    )

Distributor
 

Canadian Cryospheric Information Network

-

Polar Data Catalogue


200 University Avenue West, University of Waterloo

,

Waterloo

,

Ontario

,

N2L 3G1

,

Canada

(519) 888-4567 x32689
Included with dataset
No
File identifier
13744e95-5418-4a78-a218-fa8e25807432 XML
Metadata language

eng; CAN

Character set
UTF8
Hierarchy level
Dataset
Date stamp
2022-04-08T13:00:27
Metadata standard name

North American Profile of ISO 19115:2003

Metadata standard version

2009-01-01

Point of contact
 

Canadian Cryospheric Information Network

-

Polar Data Catalogue


200 University Avenue West, University of Waterloo

,

Waterloo

,

Ontario

,

N2L 3G1

,

Canada

(519) 888-4567 x32689
Dataset URI

https://www.polardata.ca/pdcsearch/PDCSearchDOI.jsp?doi_id=226

 
 

Overviews

overview

Spatial extent

N
S
E
W
thumbnail


Keywords

Polar Data Catalogue Thesaurus (Canada)
Arctic Chlorophyll Franklin Bay Phytoplankton Pigments

Provided by

logo

Share on social sites

Access to the portal
Read here the full details and access to the data.

Associated resources

Not available


  •  
  •  
  •