HPLC Pigment Analysis of the Phytoplankton Community in Franklin Bay
Sample collection: The sampling was part of the Canadian Arctic Shelf Exchange Study (CASES) in which CCGS Amundsen was frozen in Franklin Bay in the coastal Beaufort Sea (Station FB/200) during winter. Upper mixed-layer microbial plankton communities were sampled 5m below the water surface using the ship CTD rosette system equipped with 12L Niskins during openwater conditions. During the time that the ship was frozen in Franklin Bay from December 2003 to early June 2004, samples were taken using a 5L Niskin bottle from 3m below the bottom ice through a 300mm hole that had been drilled 500m upstream of the ship. HPLC pigment analysis: One to two liter samples of water were filtered onto Whatman GF/F filters and stored frozen at -80C until analysis. Phytoplankton pigments on the GF/Fs were extracted in 3mL of 95 percent MeOH and 100 uL of the extracts was injected into a Varian ProStar HPLC equipped with a Symmetry C8 column. The HPLC peaks were detected by diode-array spectroscopy (350-750nm) and absorbance chromatograms were obtained at 440 (for chls) and 450nm (for carotenoids). Chlorophylls were also detected by fluorescence (excitation: 440nm; emission: 650nm). The HPLC solvent protocol was based on gradient dilution with two solvent mixtures (Zapata et al. 2000): a methanol, acetonitrile, and aqueous pyridine (50:25:25 v:v:v) solution; and a methanol, acetonitrile, and acetone (20:60:20 v:v:v) solution. The flow rate was 1mL/ min, and the equilibration time was 7min.
Simple
- Date (Publication)
- 2016-01-14
- Other citation details
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Lovejoy, C., Vincent, W.F., Bonilla, S., Roy, S., Martineau, M.-J., Terrado, R., Potvin, M., Massana, R. and Pedrós-Alió, C. 2007. Distribution, phylogeny and growth of cold-adapted picoprasinophytes in arctic seas. J. Phycol. 43, 78-89.
Matsuoka, A., Larouche, P., Poulin, M., Vincent, W. and Hattori, H. 2009. Phytoplankton community adaptation to changing light levels in the southern Beaufort Sea, Canadian Arctic, Estuarine, Coastal and Shelf Sciences 82: 537-546.
- Purpose
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To obtain a detailed description of the pigments associated with the phytoplankton community. The pigment analyses from the year-long study in the coastal Beaufort Sea of the western Canadian Arctic showed the continuous prevalence of pigments associated with eukaryotic picoplankton in the green algal class Prasinophyceae. These results confirmed the presence of Micromonas observed by microscopy.
- Status
- Completed
Canadian Cryospheric Information Network
-Polar Data Catalogue
200 University Avenue West, University of Waterloo
,Waterloo
,Ontario
,N2L 3G1
,Canada
polardata.ca
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Polar Data Catalogue Thesaurus (Canada)
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Arctic
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Chlorophyll
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Franklin Bay
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Phytoplankton
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Pigments
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- Place
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Franklin Bay, Northwest Territories, Canada (LAIQA)
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- Access constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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Terms of Use of the Polar Data Catalogue: https://www.polardata.ca/pdcinput/public/termsofuse
- Metadata language
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eng; CAN
- Topic category
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- Environment
))
- Begin date
- 2003-10-19
- End date
- 2004-08-06
- Supplemental Information
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Summary: Not Applicable Research Program(s): CASES. For further information: warwick.vincent@bio.ulaval.ca warwick.vincent@bio.ula Summary: Not Applicable Research Program(s): CASES. For further information: warwick.vincent@bio.ulaval.ca
- Distribution format
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Digital file
(1.0
)
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Canadian Cryospheric Information Network
-Polar Data Catalogue
200 University Avenue West, University of Waterloo
,Waterloo
,Ontario
,N2L 3G1
,Canada
- Included with dataset
- No
- File identifier
- 13744e95-5418-4a78-a218-fa8e25807432 XML
- Metadata language
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eng; CAN
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2022-04-08T13:00:27
- Metadata standard name
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North American Profile of ISO 19115:2003
- Metadata standard version
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2009-01-01
Canadian Cryospheric Information Network
-Polar Data Catalogue
200 University Avenue West, University of Waterloo
,Waterloo
,Ontario
,N2L 3G1
,Canada
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