13744e95-5418-4a78-a218-fa8e25807432 eng; CAN Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca 2022-04-08T13:00:27 North American Profile of ISO 19115:2003 2009-01-01 https://www.polardata.ca/pdcsearch/PDCSearchDOI.jsp?doi_id=226 HPLC Pigment Analysis of the Phytoplankton Community in Franklin Bay 2016-01-14 Vincent, Warwick Martineau, Marie-Josée Lovejoy, C., Vincent, W.F., Bonilla, S., Roy, S., Martineau, M.-J., Terrado, R., Potvin, M., Massana, R. and Pedrós-Alió, C. 2007. Distribution, phylogeny and growth of cold-adapted picoprasinophytes in arctic seas. J. Phycol. 43, 78-89. Matsuoka, A., Larouche, P., Poulin, M., Vincent, W. and Hattori, H. 2009. Phytoplankton community adaptation to changing light levels in the southern Beaufort Sea, Canadian Arctic, Estuarine, Coastal and Shelf Sciences 82: 537-546. Sample collection: The sampling was part of the Canadian Arctic Shelf Exchange Study (CASES) in which CCGS Amundsen was frozen in Franklin Bay in the coastal Beaufort Sea (Station FB/200) during winter. Upper mixed-layer microbial plankton communities were sampled 5m below the water surface using the ship CTD rosette system equipped with 12L Niskins during openwater conditions. During the time that the ship was frozen in Franklin Bay from December 2003 to early June 2004, samples were taken using a 5L Niskin bottle from 3m below the bottom ice through a 300mm hole that had been drilled 500m upstream of the ship. HPLC pigment analysis: One to two liter samples of water were filtered onto Whatman GF/F filters and stored frozen at -80C until analysis. Phytoplankton pigments on the GF/Fs were extracted in 3mL of 95 percent MeOH and 100 uL of the extracts was injected into a Varian ProStar HPLC equipped with a Symmetry C8 column. The HPLC peaks were detected by diode-array spectroscopy (350-750nm) and absorbance chromatograms were obtained at 440 (for chls) and 450nm (for carotenoids). Chlorophylls were also detected by fluorescence (excitation: 440nm; emission: 650nm). The HPLC solvent protocol was based on gradient dilution with two solvent mixtures (Zapata et al. 2000): a methanol, acetonitrile, and aqueous pyridine (50:25:25 v:v:v) solution; and a methanol, acetonitrile, and acetone (20:60:20 v:v:v) solution. The flow rate was 1mL/ min, and the equilibration time was 7min. To obtain a detailed description of the pigments associated with the phytoplankton community. The pigment analyses from the year-long study in the coastal Beaufort Sea of the western Canadian Arctic showed the continuous prevalence of pigments associated with eukaryotic picoplankton in the green algal class Prasinophyceae. These results confirmed the presence of Micromonas observed by microscopy. Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca polardata.ca http https://metadata.arice-h2020.eu/geonetwork/srv/api/records/13744e95-5418-4a78-a218-fa8e25807432/attachments/map.png Arctic Chlorophyll Franklin Bay Phytoplankton Pigments Polar Data Catalogue Thesaurus (Canada) 2021-06-02 Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca polardata.ca http https://www.polardata.ca/pdcinput/public/keywordlibrary Franklin Bay, Northwest Territories, Canada (LAIQA) Terms of Use of the Polar Data Catalogue: https://www.polardata.ca/pdcinput/public/termsofuse eng; CAN environment -126.27 -126.27 69.95 69.95 Time period can be either covered by the content of the dataset or the date when the data has been collected. 2003-10-19 2004-08-06 Summary: Not Applicable Research Program(s): CASES. For further information: warwick.vincent@bio.ulaval.ca false Digital file 1.0 Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca This metadata was generated from the PDC metadata database at 2021-06-02 Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca