This study is based on sampling conducted partially within the framework of the (1) Arctic Biological Station program-Biological Oceanography Section (ABS-BOS) from 1973 to 1975, (2) CCGS Sir Wilfrid Laurier program and CASES from 2002 to 2004, (3) IPY-CFL from 2007 to 2008, (4) through research collaborations among the CCGS Amundsen program, ArcticNet, BP Exploration Operating Company Limited, ExxonMobil and Imperial Oil from 2009 to 2011, and (5) BREA in 2012. Macrobenthos communities were sampled at 235 stations from 1973 to 2012 between April and November through different scientific programs and onboard different research vessels. Faunal samples were collected mostly with a USNEL box corer (0.25 m2), except from 1973 to 2002 where different grab models were used. Due to shared sediment requirements, on average 0.12 ± 0.05 m2 of sediment were sieved from each box core or grab sample. All box core and grab catches were washed under running seawater onboard over a 0.4 mm sieve during the CASES program and over a 0.5 mm sieve during all other programs, resulting in all macrobenthic invertebrates considered here being ≥ 0.5 mm size. Taxa were preserved in a 4 to 5 % seawater-formaldehyde solution buffered with sodium tetraethylborate for later identification in the lab and then transferred in 70 % ethanol for long-term storage.

A video-survey using a Super Mohawk remotely operated vehicle (ROV) was conducted in August 2013 in the Disko Fan area, SE Baffin Bay. A multibeam survey was conducted in the area before the ROV dive. The benthic environment was video-recorded using a standard-definition color camera, at depths of ~900-950 m.

Benthic grab samples and underwater towed video samples were collected at long-term ecology sites while onboard the Government of Nunavut vessel MV Nulialjuk and CCGS Amundsen in July and October 2016. On-board the MV Nulialjuk, benthic grab samples (triplicate samples) and underwater towed video samples were collected at 5 long-term ecology stations near Iqaluit. An additional 7 benthic grab samples (triplicate samples) and towed video samples were collected near Cairn Island. On-board the CCGS Amundsen, benthic grab samples were collected at two long-term ecology stations (triplicate samples) near Cairn Island. Sampling gear included a Van Veen grab sampler, box core, and HD GoPro video system. Initial observations suggest that these stations are predominantly muddy sand with some larger cobbles present. Towed video analysis and benthic grab samples indicate the presence of the algae Laminaria sp. and Agarum clathratum and bivalves Mya truncata, Yoldia hyperborea, Clinocardium ciliatum, and Serripes groenlandicus. Ophiuroid echinoderms and tubiculous polychaete worms and tubes are abundant. Once all benthic grab samples and towed video samples are processed, this data will be compared with the historic sample data to answer questions about the nature of long-term change of the benthos at this region in inner Frobisher Bay.

Data were mainly collected from the CCGS Amundsen (2015 to 2019) and from the William-Kennedy (2019). An Agassiz trawl (1.5 m width × 0.7 m height, cod end of 0.5 cm mesh size) was towed on the seabed at a speed of 1.5-2 knots for 3 to 5 minutes to survey epibenthic species diversity, abundance, and biomass. Retrieved samples were washed with seawater in a sieve (2 mm mesh), and organisms were sorted and identified to the lowest taxonomical level possible. Each taxon was counted, and biomass was measured. A box corer was deployed to quantitatively sample diversity, abundance, and biomass of endobenthic organisms (macrobenthos > 0.5 mm). After retrieval of the box corer, a subsample of 0.125 m2 area and 12-15 cm depth was collected and passed through a 0.5 mm mesh sieve to separate sediment from endofauna. Organisms were immediately preserved in a 4% buffered formaldehyde solution for further taxonomical identification in the laboratory.

Multibeam echosounder data near Qikiqtarjuaq were collected by the CCGS Amundsen in 2007 and by the RV Nuliajuk from 2012-2015. Data collected between 2007 and 2014 were processed by the Ocean Mapping Group at the University of New Brunswick (http://www.omg.unb.ca/) and were retrieved from their data sharing interface (http://www.omg.unb.ca/Projects/Arctic/SE_Baffin/). Data collected in 2015 were processed by the Fisheries and Marine Institute at Memorial University of Newfoundland (https://www.mi.mun.ca/) and were obtained directly from them. These datasets were clipped to only include the area near Qikiqtarjuaq relevant to the benthic habitat mapping project lead by the Memorial University Marine Habitat Mapping Group. Individual datasets were mosaicked to create single continuous bathymetry (m) and backscatter (dB) raster layers at 5 m resolution. Mapping activities were funded by ArcticNet and the Government of Nunavut, Department of Environment, Fisheries and Sealing Division to support benthic habitat mapping for marine ecosystem management in this area.

Video surveys were conducted in July 2016 aboard CCGS Amundsen, using a Super Mohawk remotely operated vehicle (ROV) at Hatton Basin, Labrador (Canada). ROV dives occurred at two nearby sites (~28 km apart) in the Hatton Basin area (Dives 50 and 51). The benthic environment was video-recorded and photographed using a high-definition camera (1Cam Alpha, Sub C Imaging, 24.1 megapixels) mounted on the ROV, at depths ranging between 556-633 m. ROV transects were conducted to collect video data, and samples of corals and sponges were also collected during dive 51. Box-cores were deployed at the two sites. Sea water was collected at several depth intervals from surface to near-bottom to characterize particulate organic matter and to determine calcium carbonate saturation.

A video-survey using a Super Mohawk remotely operated vehicle (ROV) was conducted in October 2015 in a site near Cape Dyer, Nunavut. The benthic environment was video-recorded and opportunistically photographed using a high-definition camera (1Cam Alpha, Sub C Imaging, 24.1 megapixels), at a depth of ~750 m along a transect line 935 m long.

A video-survey using a Super Mohawk remotely operated vehicle (ROV) was conducted in October 2015 at Inner Frobisher Bay, Nunavut. The benthic environment was video-recorded and opportunistically photographed using a high-definition camera (1Cam Alpha, Sub C Imaging, 24.1 megapixels), at a depth of ~59-139 m along a transect line 1.2 km long.

A video-survey using a Super Mohawk remotely operated vehicle (ROV) was conducted in October 2015 near Qikiqtarjuaq, Nunavut. The benthic environment was video-recorded and opportunistically photographed using a high-definition camera (1Cam Alpha, Sub C Imaging, 24.1 megapixels), at a depth of ~620-680 m along a transect line 954m long.

A first survey was conducted in and around EL446 onboard the CCGS Amundsen from 16 July to 30 July, 2009. A total of 21 biophysical stations were sampled, involving 25 box core deployments and 18 Agassiz trawl tows. In addition, the Remotely Operated Vehicle (ROV) was deployed once (station 10) for epibenthos observation. A second survey was conducted in EL449 and EL451 onboard the CCGS Amundsen from 12 August to 26 August, 2010. A total of 18 biophysical stations were sampled, comprising 25 box core deployments and 18 Agassiz trawl tows. A third survey was conducted in EL451 and EL453 onboard the CCGS Amundsen from 7 September to 22 September 2011. A total of 13 biophysical stations were sampled, comprising 18 box core deployments and 13 Agassiz trawl tows. The box core was deployed to quantitatively sample diversity and abundance of endobenthic organisms. After retrieval of the box core, a subsample of about 0.125 m2 area and 12-15 cm depth was collected and passed through a 0.5 mm mesh sieve to separate sediment from endofauna. Organisms were immediately preserved in a 4% buffered formaldehyde solution for further taxonomical identification in the laboratory. The volume of sediments sieved from each box core was measured (depth × width × length) to the nearest 1 cm to estimate endobenthic fauna density in each sample. An Agassiz trawl (1.5 m width × 0.7 m height, cod end of 0.5 cm mesh size) was towed on the seabed at a speed of 1.5 - 2 knots for about 3 to 5 minutes to survey epibenthic species diversity and abundance. Retrieved samples were washed with seawater in a sieve (0.5 mm mesh), and organisms were counted and identified to the lowest taxonomical level possible.