32e313e0-7a0d-411c-8849-82b47c881f6f eng; CAN Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca 2022-04-08T13:00:30 North American Profile of ISO 19115:2003 2009-01-01 https://www.polardata.ca/pdcsearch/PDCSearchDOI.jsp?doi_id=13145 Biodiversity and Gene Expression of Microbial Communities in the Canadian Arctic 2020-03-13 Lovejoy, Connie Thaler, Mary Lovejoy, Connie Coltriano, David Freyria, Nastasia Galand, Pierre Joli, Nathalie Kalenitchenko, Dimitri Monier, Adam Thaler, M., Freyria, N., Kalenitchenko, D., Galand, P., Monier, A., Coltriano, D., Joli, N., & Lovejoy, C. (2020). Biodiversity and Gene Expression of Microbial Communities in the Canadian Arctic. Waterloo, Canada: Canadian Cryospheric Information Network (CCIN). (Unpublished Data). As part of the ArcticNet expeditions (http://www.arcticnet.ulaval.ca) on the CCGS Amundsen in 2008-2019, we collected water samples from stations throughout the Canadian Arctic. Cruise tracks varied from year to year, but always included a transect of northern Baffin Bay, and sometimes sampling in Lancaster Sound, the Beaufort Sea, and/or along the east coast of Baffin Island. The sampling period ranged from summer to autumn. Samples were collected by CTD-rosette from 2-8 depths corresponding to water column features such as surface water, the subsurface chlorophyll maximum, the nitracline, or Atlantic Water. To collect samples for DNA/RNA, water was filtered through 3-µm filters and 0.2 µm cartridges, which were conserved in a buffer at -80°C. These have been used for high-throughput sequencing for amplicon-based surveys, metagenomics, and other molecular techniques. Samples for flow cytometry (to enumerate bacterial and/or pigmented cells), microscopy (with a fluorescent DAPI stain, or using FNU preservative for identifying larger cells), fluorescent in-situ hybridization (another microscopy technique), chlorophyll a (to quantify photosynthetic organisms), and HPLC pigment analysis (to identify different algae taxa), were also collected. Samples from which nucleic acids have been extracted and sequenced in many projects. Samples from which nucleic acids have been extracted and sequenced can be found in the GenBank Short Read Archive under the project accession numbers PRJNA202104, PRJNA283142, PRJNA283296, PRJNA383398, and SRX037894-SRX037896 These samples were collected aboard the CCGS Amundsen as part of a long-term survey of the distribution and diversity of microbial communities in the Canadian Arctic. Each year we returned to critical areas for productivity in the Arctic Ocean, including the North Water polynya region of Baffin Bay, Lancaster Sound, while sampling stations in other regions may be added on an opportunistic basis. The multi-year nature of this survey makes it particularly useful for addressing questions about changes in microbial communities in response to changing conditions in the Arctic. Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca polardata.ca http https://metadata.arice-h2020.eu/geonetwork/srv/api/records/32e313e0-7a0d-411c-8849-82b47c881f6f/attachments/map.png Bacteria Baffin Bay Beaufort Sea Canadian Arctic Archipelago DNA Marine ecology Phytoplankton Protists RNA Polar Data Catalogue Thesaurus (Canada) 2021-06-02 Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca polardata.ca http https://www.polardata.ca/pdcinput/public/keywordlibrary Arctic Ocean, Canada - NAABK Terms of Use of the Polar Data Catalogue: https://www.polardata.ca/pdcinput/public/termsofuse eng; CAN environment -140.7963 -51.8733 62.3845 81.7535 Time period can be either covered by the content of the dataset or the date when the data has been collected. 2008-07-20 2019-08-11 Summary: Not Applicable Research Program(s): IPY,Amundsen Science,ArcticNet,TAKUVIK - Ocean. For further information: connie.lovejoy@bio.ulaval.ca false Digital file 1.0 Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca This metadata was generated from the PDC metadata database at 2021-06-02 Polar Data Catalogue Canadian Cryospheric Information Network (519) 888-4567 x32689 200 University Avenue West, University of Waterloo Waterloo Ontario N2L 3G1 Canada pdc@uwaterloo.ca