This study is based on sampling conducted partially within the framework of the (1) Arctic Biological Station program-Biological Oceanography Section (ABS-BOS) from 1973 to 1975, (2) CCGS Sir Wilfrid Laurier program and CASES from 2002 to 2004, (3) IPY-CFL from 2007 to 2008, (4) through research collaborations among the CCGS Amundsen program, ArcticNet, BP Exploration Operating Company Limited, ExxonMobil and Imperial Oil from 2009 to 2011, and (5) BREA in 2012.

Macrobenthos communities were sampled at 235 stations from 1973 to 2012 between April and November through different scientific programs and onboard different research vessels. Faunal samples were collected mostly with a USNEL box corer (0.25 m2), except from 1973 to 2002 where different grab models were used. Due to shared sediment requirements, on average 0.12 ± 0.05 m2 of sediment were sieved from each box core or grab sample. All box core and grab catches were washed under running seawater onboard over a 0.4 mm sieve during the CASES program and over a 0.5 mm sieve during all other programs, resulting in all macrobenthic invertebrates considered here being ≥ 0.5 mm size.

Taxa were preserved in a 4 to 5 % seawater-formaldehyde solution buffered with sodium tetraethylborate for later identification in the lab and then transferred in 70 % ethanol for long-term storage.